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qRT-PCR was performed in QuantStudio 3 realtime PCR system (Applied Biosystems, USA) using ChamQ SYBR qPCR Master Mix (Low ROX Premixed) (Q331-02, Vazyme)

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An experimental study showed that in the mature genetic model of type 2 diabetes, the db/db mouse, after systemic administration of the iron chelator deferoxamine by intraperitoneal injection for 12 weeks, significant reductions in inflammatory markers such as IL-1 and TNF- were observed, and retinal electrophysiological function was improved

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Its especially effective when paired with BPC-157 (also known as the Wolverine Blend when combining BPC-157 and TB-500) for faster recovery from injuries